Identification of novel Sinorhizobium meliloti mutants compromised for oxidative stress protection and symbiosis.
نویسندگان
چکیده
Employing a novel two-part screen, we identified Sinorhizobium meliloti mutants that were both sensitive to hydrogen peroxide and symbiotically defective on the host plant Medicago sativa. The mutations affect a wide variety of cellular processes and represent both novel and previously identified genes important in symbiosis.
منابع مشابه
Disruption of sitA compromises Sinorhizobium meliloti for manganese uptake required for protection against oxidative stress.
During the initial stages of symbiosis with the host plant Medicago sativa, Sinorhizobium meliloti must overcome an oxidative burst produced by the plant in order for proper symbiotic development to continue. While identifying mutants defective in symbiosis and oxidative stress defense, we isolated a mutant with a transposon insertion mutation of sitA, which encodes the periplasmic binding prot...
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متن کاملRegulatory and DNA repair genes contribute to the desiccation resistance of Sinorhizobium meliloti Rm1021.
Sinorhizobium meliloti can form a nitrogen-fixing symbiotic relationship with alfalfa after bacteria in the soil infect emerging root hairs of the growing plant. To be successful at this, the bacteria must be able to survive in the soil between periods of active plant growth, including when conditions are dry. The ability of S. meliloti to withstand desiccation has been known for years, but gen...
متن کاملOnly one of five groEL genes is required for viability and successful symbiosis in Sinorhizobium meliloti.
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متن کاملThe novel genes emmABC are associated with exopolysaccharide production, motility, stress adaptation, and symbiosis in Sinorhizobium meliloti.
The nitrogen-fixing symbiont Sinorhizobium meliloti senses and responds to constantly changing environmental conditions as it makes its way through the soil in search of its leguminous plant host, Medicago sativa (alfalfa). As a result, this bacterium regulates various aspects of its physiology in order to respond appropriately to stress, starvation, and competition. For example, exopolysacchar...
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 189 5 شماره
صفحات -
تاریخ انتشار 2007